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goscripttm qpcr master mix kit (promega, madison, wi, usa)  (Promega)

 
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    Promega goscripttm qpcr master mix kit (promega, madison, wi, usa)
    TFP reduces BBB disruption and improves stroke outcomes after IS (A) Western blotting analysis of CaM protein expression levels in WT mice after I/R followed by treatment with TFP at different concentrations (2, 5, 10, and 20 mg/kg). (B) Quantitative analysis of the expression levels of CaM in (A) (n = 3 per group). (C) Representative brain sections stained with TTC. (D) Infarct volume (n = 6). (E) mNSS score (n = 6). (F) Representative immunofluorescence images from EB-leakage tests taken before and after I/R followed by treatment with TFP in WT mice (scale bar, 50 mm). (G and H) Quantitative analysis of the radiant efficiency of EB in (F) (n = 3 per group). (I) Representative immunofluorescence images of ZO-1 or claudin-5 (red), CD31 (green), and DAPI (blue) staining of ischemic and normal brain areas in WT mice after I/R (scale bars, 50 μm). (J and K) Quantitative analysis of CD31 + ZO-1 + and CD31 + claudin-5 + cells as a percentage of CD31 + cells in (I) (n = 6 per group). (L–N) <t>RT-qPCR</t> verification of the mRNA levels of claudin-5, occludin, and ZO-1 in WT mice after I/R followed by treatment with TFP (n = 3 per group). (O) Western blotting analysis of ZO-1, occludin, and claudin-5 in WT mice after I/R followed by TFP treatment. (P) Quantitative analysis of the expression levels of ZO-1, occludin, and claudin-5 in (O) (n = 3 per group). Data are represented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01.
    Goscripttm Qpcr Master Mix Kit (Promega, Madison, Wi, Usa), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goscripttm+qpcr+master+mix/pmc10910233-283-1-6?v=Promega
    Average 90 stars, based on 1 article reviews
    goscripttm qpcr master mix kit (promega, madison, wi, usa) - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Trifluoperazine regulates blood-brain barrier permeability via the MLCK/p-MLC pathway to promote ischemic stroke recovery"

    Article Title: Trifluoperazine regulates blood-brain barrier permeability via the MLCK/p-MLC pathway to promote ischemic stroke recovery

    Journal: iScience

    doi: 10.1016/j.isci.2024.109156

    TFP reduces BBB disruption and improves stroke outcomes after IS (A) Western blotting analysis of CaM protein expression levels in WT mice after I/R followed by treatment with TFP at different concentrations (2, 5, 10, and 20 mg/kg). (B) Quantitative analysis of the expression levels of CaM in (A) (n = 3 per group). (C) Representative brain sections stained with TTC. (D) Infarct volume (n = 6). (E) mNSS score (n = 6). (F) Representative immunofluorescence images from EB-leakage tests taken before and after I/R followed by treatment with TFP in WT mice (scale bar, 50 mm). (G and H) Quantitative analysis of the radiant efficiency of EB in (F) (n = 3 per group). (I) Representative immunofluorescence images of ZO-1 or claudin-5 (red), CD31 (green), and DAPI (blue) staining of ischemic and normal brain areas in WT mice after I/R (scale bars, 50 μm). (J and K) Quantitative analysis of CD31 + ZO-1 + and CD31 + claudin-5 + cells as a percentage of CD31 + cells in (I) (n = 6 per group). (L–N) RT-qPCR verification of the mRNA levels of claudin-5, occludin, and ZO-1 in WT mice after I/R followed by treatment with TFP (n = 3 per group). (O) Western blotting analysis of ZO-1, occludin, and claudin-5 in WT mice after I/R followed by TFP treatment. (P) Quantitative analysis of the expression levels of ZO-1, occludin, and claudin-5 in (O) (n = 3 per group). Data are represented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01.
    Figure Legend Snippet: TFP reduces BBB disruption and improves stroke outcomes after IS (A) Western blotting analysis of CaM protein expression levels in WT mice after I/R followed by treatment with TFP at different concentrations (2, 5, 10, and 20 mg/kg). (B) Quantitative analysis of the expression levels of CaM in (A) (n = 3 per group). (C) Representative brain sections stained with TTC. (D) Infarct volume (n = 6). (E) mNSS score (n = 6). (F) Representative immunofluorescence images from EB-leakage tests taken before and after I/R followed by treatment with TFP in WT mice (scale bar, 50 mm). (G and H) Quantitative analysis of the radiant efficiency of EB in (F) (n = 3 per group). (I) Representative immunofluorescence images of ZO-1 or claudin-5 (red), CD31 (green), and DAPI (blue) staining of ischemic and normal brain areas in WT mice after I/R (scale bars, 50 μm). (J and K) Quantitative analysis of CD31 + ZO-1 + and CD31 + claudin-5 + cells as a percentage of CD31 + cells in (I) (n = 6 per group). (L–N) RT-qPCR verification of the mRNA levels of claudin-5, occludin, and ZO-1 in WT mice after I/R followed by treatment with TFP (n = 3 per group). (O) Western blotting analysis of ZO-1, occludin, and claudin-5 in WT mice after I/R followed by TFP treatment. (P) Quantitative analysis of the expression levels of ZO-1, occludin, and claudin-5 in (O) (n = 3 per group). Data are represented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01.

    Techniques Used: Disruption, Western Blot, Expressing, Staining, Immunofluorescence, Quantitative RT-PCR

    TFP represses CaM expression levels and decreases BBB breakdown after OGD (A and B) FITC-dextran transport assay showing the permeability of bEnd.3 cells treated with TFP before and after OGD (n = 3 per group). (C) Western blotting analysis of CaM protein expression levels in bEnd.3 cells treated with TFP after OGD. (D) Quantitative analysis of the expression levels of CaM in (C) (n = 3 per group). (E) Representative immunofluorescence images of TJ proteins (claudin-5, occludin, and ZO-1) in bEnd.3 cells subjected to OGD (scale bar, 20 μm). (F–H) Quantitative evaluation of the fluorescence intensity of claudin-5, occludin, and ZO-1 in (E) (n = 6 per group). (I–K) RT-qPCR verification of the mRNA levels of claudin-5, occludin, and ZO-1 in bEnd.3 cells after OGD followed by treatment with TFP (n = 3 per group). (L) Western blotting analysis of ZO-1, occludin, and claudin-5 in bEnd.3 cells treated with TFP after OGD. (M) Quantitative analysis of the expression levels of ZO-1, occludin, and claudin-5 in (L) (n = 3 per group). Data are represented as mean ± SEM. ns, p > 0.05, ∗∗p < 0.01.
    Figure Legend Snippet: TFP represses CaM expression levels and decreases BBB breakdown after OGD (A and B) FITC-dextran transport assay showing the permeability of bEnd.3 cells treated with TFP before and after OGD (n = 3 per group). (C) Western blotting analysis of CaM protein expression levels in bEnd.3 cells treated with TFP after OGD. (D) Quantitative analysis of the expression levels of CaM in (C) (n = 3 per group). (E) Representative immunofluorescence images of TJ proteins (claudin-5, occludin, and ZO-1) in bEnd.3 cells subjected to OGD (scale bar, 20 μm). (F–H) Quantitative evaluation of the fluorescence intensity of claudin-5, occludin, and ZO-1 in (E) (n = 6 per group). (I–K) RT-qPCR verification of the mRNA levels of claudin-5, occludin, and ZO-1 in bEnd.3 cells after OGD followed by treatment with TFP (n = 3 per group). (L) Western blotting analysis of ZO-1, occludin, and claudin-5 in bEnd.3 cells treated with TFP after OGD. (M) Quantitative analysis of the expression levels of ZO-1, occludin, and claudin-5 in (L) (n = 3 per group). Data are represented as mean ± SEM. ns, p > 0.05, ∗∗p < 0.01.

    Techniques Used: Expressing, Transport Assay, Permeability, Western Blot, Immunofluorescence, Fluorescence, Quantitative RT-PCR



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    goscripttm qpcr master mix kit (promega, madison, wi, usa)  (Promega)
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    Promega goscripttm qpcr master mix kit (promega, madison, wi, usa)
    TFP reduces BBB disruption and improves stroke outcomes after IS (A) Western blotting analysis of CaM protein expression levels in WT mice after I/R followed by treatment with TFP at different concentrations (2, 5, 10, and 20 mg/kg). (B) Quantitative analysis of the expression levels of CaM in (A) (n = 3 per group). (C) Representative brain sections stained with TTC. (D) Infarct volume (n = 6). (E) mNSS score (n = 6). (F) Representative immunofluorescence images from EB-leakage tests taken before and after I/R followed by treatment with TFP in WT mice (scale bar, 50 mm). (G and H) Quantitative analysis of the radiant efficiency of EB in (F) (n = 3 per group). (I) Representative immunofluorescence images of ZO-1 or claudin-5 (red), CD31 (green), and DAPI (blue) staining of ischemic and normal brain areas in WT mice after I/R (scale bars, 50 μm). (J and K) Quantitative analysis of CD31 + ZO-1 + and CD31 + claudin-5 + cells as a percentage of CD31 + cells in (I) (n = 6 per group). (L–N) <t>RT-qPCR</t> verification of the mRNA levels of claudin-5, occludin, and ZO-1 in WT mice after I/R followed by treatment with TFP (n = 3 per group). (O) Western blotting analysis of ZO-1, occludin, and claudin-5 in WT mice after I/R followed by TFP treatment. (P) Quantitative analysis of the expression levels of ZO-1, occludin, and claudin-5 in (O) (n = 3 per group). Data are represented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01.
    Goscripttm Qpcr Master Mix Kit (Promega, Madison, Wi, Usa), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goscripttm+qpcr+master+mix/pmc10910233-283-1-6?v=Promega
    Average 90 stars, based on 1 article reviews
    goscripttm qpcr master mix kit (promega, madison, wi, usa) - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier
    goscripttm qpcr master mix kit  (Promega)
    90
    Promega goscripttm qpcr master mix kit
    TFP reduces BBB disruption and improves stroke outcomes after IS (A) Western blotting analysis of CaM protein expression levels in WT mice after I/R followed by treatment with TFP at different concentrations (2, 5, 10, and 20 mg/kg). (B) Quantitative analysis of the expression levels of CaM in (A) (n = 3 per group). (C) Representative brain sections stained with TTC. (D) Infarct volume (n = 6). (E) mNSS score (n = 6). (F) Representative immunofluorescence images from EB-leakage tests taken before and after I/R followed by treatment with TFP in WT mice (scale bar, 50 mm). (G and H) Quantitative analysis of the radiant efficiency of EB in (F) (n = 3 per group). (I) Representative immunofluorescence images of ZO-1 or claudin-5 (red), CD31 (green), and DAPI (blue) staining of ischemic and normal brain areas in WT mice after I/R (scale bars, 50 μm). (J and K) Quantitative analysis of CD31 + ZO-1 + and CD31 + claudin-5 + cells as a percentage of CD31 + cells in (I) (n = 6 per group). (L–N) <t>RT-qPCR</t> verification of the mRNA levels of claudin-5, occludin, and ZO-1 in WT mice after I/R followed by treatment with TFP (n = 3 per group). (O) Western blotting analysis of ZO-1, occludin, and claudin-5 in WT mice after I/R followed by TFP treatment. (P) Quantitative analysis of the expression levels of ZO-1, occludin, and claudin-5 in (O) (n = 3 per group). Data are represented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01.
    Goscripttm Qpcr Master Mix Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    goscripttm qpcr master mix  (Promega)
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    Promega goscripttm qpcr master mix
    TFP reduces BBB disruption and improves stroke outcomes after IS (A) Western blotting analysis of CaM protein expression levels in WT mice after I/R followed by treatment with TFP at different concentrations (2, 5, 10, and 20 mg/kg). (B) Quantitative analysis of the expression levels of CaM in (A) (n = 3 per group). (C) Representative brain sections stained with TTC. (D) Infarct volume (n = 6). (E) mNSS score (n = 6). (F) Representative immunofluorescence images from EB-leakage tests taken before and after I/R followed by treatment with TFP in WT mice (scale bar, 50 mm). (G and H) Quantitative analysis of the radiant efficiency of EB in (F) (n = 3 per group). (I) Representative immunofluorescence images of ZO-1 or claudin-5 (red), CD31 (green), and DAPI (blue) staining of ischemic and normal brain areas in WT mice after I/R (scale bars, 50 μm). (J and K) Quantitative analysis of CD31 + ZO-1 + and CD31 + claudin-5 + cells as a percentage of CD31 + cells in (I) (n = 6 per group). (L–N) <t>RT-qPCR</t> verification of the mRNA levels of claudin-5, occludin, and ZO-1 in WT mice after I/R followed by treatment with TFP (n = 3 per group). (O) Western blotting analysis of ZO-1, occludin, and claudin-5 in WT mice after I/R followed by TFP treatment. (P) Quantitative analysis of the expression levels of ZO-1, occludin, and claudin-5 in (O) (n = 3 per group). Data are represented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01.
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    Image Search Results


    TFP reduces BBB disruption and improves stroke outcomes after IS (A) Western blotting analysis of CaM protein expression levels in WT mice after I/R followed by treatment with TFP at different concentrations (2, 5, 10, and 20 mg/kg). (B) Quantitative analysis of the expression levels of CaM in (A) (n = 3 per group). (C) Representative brain sections stained with TTC. (D) Infarct volume (n = 6). (E) mNSS score (n = 6). (F) Representative immunofluorescence images from EB-leakage tests taken before and after I/R followed by treatment with TFP in WT mice (scale bar, 50 mm). (G and H) Quantitative analysis of the radiant efficiency of EB in (F) (n = 3 per group). (I) Representative immunofluorescence images of ZO-1 or claudin-5 (red), CD31 (green), and DAPI (blue) staining of ischemic and normal brain areas in WT mice after I/R (scale bars, 50 μm). (J and K) Quantitative analysis of CD31 + ZO-1 + and CD31 + claudin-5 + cells as a percentage of CD31 + cells in (I) (n = 6 per group). (L–N) RT-qPCR verification of the mRNA levels of claudin-5, occludin, and ZO-1 in WT mice after I/R followed by treatment with TFP (n = 3 per group). (O) Western blotting analysis of ZO-1, occludin, and claudin-5 in WT mice after I/R followed by TFP treatment. (P) Quantitative analysis of the expression levels of ZO-1, occludin, and claudin-5 in (O) (n = 3 per group). Data are represented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01.

    Journal: iScience

    Article Title: Trifluoperazine regulates blood-brain barrier permeability via the MLCK/p-MLC pathway to promote ischemic stroke recovery

    doi: 10.1016/j.isci.2024.109156

    Figure Lengend Snippet: TFP reduces BBB disruption and improves stroke outcomes after IS (A) Western blotting analysis of CaM protein expression levels in WT mice after I/R followed by treatment with TFP at different concentrations (2, 5, 10, and 20 mg/kg). (B) Quantitative analysis of the expression levels of CaM in (A) (n = 3 per group). (C) Representative brain sections stained with TTC. (D) Infarct volume (n = 6). (E) mNSS score (n = 6). (F) Representative immunofluorescence images from EB-leakage tests taken before and after I/R followed by treatment with TFP in WT mice (scale bar, 50 mm). (G and H) Quantitative analysis of the radiant efficiency of EB in (F) (n = 3 per group). (I) Representative immunofluorescence images of ZO-1 or claudin-5 (red), CD31 (green), and DAPI (blue) staining of ischemic and normal brain areas in WT mice after I/R (scale bars, 50 μm). (J and K) Quantitative analysis of CD31 + ZO-1 + and CD31 + claudin-5 + cells as a percentage of CD31 + cells in (I) (n = 6 per group). (L–N) RT-qPCR verification of the mRNA levels of claudin-5, occludin, and ZO-1 in WT mice after I/R followed by treatment with TFP (n = 3 per group). (O) Western blotting analysis of ZO-1, occludin, and claudin-5 in WT mice after I/R followed by TFP treatment. (P) Quantitative analysis of the expression levels of ZO-1, occludin, and claudin-5 in (O) (n = 3 per group). Data are represented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01.

    Article Snippet: The GoScriptTM qPCR Master Mix Kit (Promega, Madison, WI, USA) was used for fluorescence real-time quantitative polymerase chain reaction (RT-qPCR) assays, according to the manufacturer’s instructions.

    Techniques: Disruption, Western Blot, Expressing, Staining, Immunofluorescence, Quantitative RT-PCR

    TFP represses CaM expression levels and decreases BBB breakdown after OGD (A and B) FITC-dextran transport assay showing the permeability of bEnd.3 cells treated with TFP before and after OGD (n = 3 per group). (C) Western blotting analysis of CaM protein expression levels in bEnd.3 cells treated with TFP after OGD. (D) Quantitative analysis of the expression levels of CaM in (C) (n = 3 per group). (E) Representative immunofluorescence images of TJ proteins (claudin-5, occludin, and ZO-1) in bEnd.3 cells subjected to OGD (scale bar, 20 μm). (F–H) Quantitative evaluation of the fluorescence intensity of claudin-5, occludin, and ZO-1 in (E) (n = 6 per group). (I–K) RT-qPCR verification of the mRNA levels of claudin-5, occludin, and ZO-1 in bEnd.3 cells after OGD followed by treatment with TFP (n = 3 per group). (L) Western blotting analysis of ZO-1, occludin, and claudin-5 in bEnd.3 cells treated with TFP after OGD. (M) Quantitative analysis of the expression levels of ZO-1, occludin, and claudin-5 in (L) (n = 3 per group). Data are represented as mean ± SEM. ns, p > 0.05, ∗∗p < 0.01.

    Journal: iScience

    Article Title: Trifluoperazine regulates blood-brain barrier permeability via the MLCK/p-MLC pathway to promote ischemic stroke recovery

    doi: 10.1016/j.isci.2024.109156

    Figure Lengend Snippet: TFP represses CaM expression levels and decreases BBB breakdown after OGD (A and B) FITC-dextran transport assay showing the permeability of bEnd.3 cells treated with TFP before and after OGD (n = 3 per group). (C) Western blotting analysis of CaM protein expression levels in bEnd.3 cells treated with TFP after OGD. (D) Quantitative analysis of the expression levels of CaM in (C) (n = 3 per group). (E) Representative immunofluorescence images of TJ proteins (claudin-5, occludin, and ZO-1) in bEnd.3 cells subjected to OGD (scale bar, 20 μm). (F–H) Quantitative evaluation of the fluorescence intensity of claudin-5, occludin, and ZO-1 in (E) (n = 6 per group). (I–K) RT-qPCR verification of the mRNA levels of claudin-5, occludin, and ZO-1 in bEnd.3 cells after OGD followed by treatment with TFP (n = 3 per group). (L) Western blotting analysis of ZO-1, occludin, and claudin-5 in bEnd.3 cells treated with TFP after OGD. (M) Quantitative analysis of the expression levels of ZO-1, occludin, and claudin-5 in (L) (n = 3 per group). Data are represented as mean ± SEM. ns, p > 0.05, ∗∗p < 0.01.

    Article Snippet: The GoScriptTM qPCR Master Mix Kit (Promega, Madison, WI, USA) was used for fluorescence real-time quantitative polymerase chain reaction (RT-qPCR) assays, according to the manufacturer’s instructions.

    Techniques: Expressing, Transport Assay, Permeability, Western Blot, Immunofluorescence, Fluorescence, Quantitative RT-PCR